What are the necessary conditions to ensure Elisa test results? -Huaqiang Electronic Network

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Elisa detection method is fast and simple, and has become the first choice for testing by many research institutes. How to operate correctly is a necessary condition to ensure the accuracy and reliability of ELISA test results. In this regard, Shanghai Jinma Bio has detailed analysis of the operation steps from the aspects of sample loading and color development:
The first is the sample analysis: Our company proposes to put people in the incubator in time after the sample is added. When there are more specimens, it is necessary to operate in batches. According to the instructions, the operation time is strictly controlled to prevent the incubation time from being artificially extended, resulting in non-specific binding surrounding the reaction well, which is difficult to clean thoroughly.
Secondly, for the Elisa test, the color developer should be prepared as far as possible before use. It is not necessary to use the expired color developer, and the light blue TMB color developer can be seen by the naked eye.
However, after adding the enzyme reagent, we wipe it on the surface of the microplate with absorbent paper. Shanghai Jinma prompts Elisa to test the results accurately, because the reasonable amount of detection, so as not to cause too much reaction plate to cause long washing time.
Thank you for your support and encouragement to Shanghai Jinma Bio, I wish you a happy shopping!